Oligonucleotide Fingerprinting of
Clone Libraries
Richard Mott, Sebastian Meier-Ewart and Hans Lehrach
Genome Analysis Laboratory
Imperial Cancer Research Fund
London WC2A 3PX
We describe computational aspects of fingerprinting clone libraries
(principally cDNAs), using data from the hybridisation of short
oligonucleotide probes onto nylon filters which contain clones arrayed
at high density. The topics covered are:
- Selection of probes
- Image analysis of filters
- Assesment of hybridisation quality using control clones
- Matching observed fingerprints of clones with known sequence to their
expected fingerprints
- Clustering of cDNA clones
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Document last modified on March 28, 2000.