Student Interface to the Biology Workbench:

http://bighorn.animal.uiuc.edu/cgi-bin/sib.py

1.	Create a username and password and log in.

2.	Create a new session named "Day 1"

3.	Click "Nucleic Tools" button at the top of the page

4.	In the "Ndjinn" box, type "Hemoglobin" and select the "Genbank Primate Sequences" database

5.	Select the "Human beta globin region on chromosome 11." checkbox and import the sequence

6.	Why not view your sequence. Select your sequence at the bottom of the page and click the "View" button. Click "Return" when you are suitably impressed.

7.	This should return you to the nucleic acids tool page. Let's find out if there are any plants which have something like our hemoglobin. Check your imported sequence at the bottom of the page, and then back up by the "BLASTN" button (which is about 1/4 way down the page) select the "GenBank Plant Seqeunces" database, and then click the "BLASTN" button.

8.	Scroll down a bit to see a table of matches, and then scroll down below the table to see the portions of the discovered sequences which matched. For extra credit, please tell me what that Nicotiana tabacum cDNA-AFLP-fragment is doing being so similar to our hemoglobin. For a bit more extra credit, compute how many nucleotides you would need to generate at random before finding a match that is that good.

9.	Scroll back up to the top of that table and check the two Nicotiana tabacum cDNA-AFLP-fragment sequences, and scroll up a bit again to import those sequences.

10.	Back on your Nucleic acids tool page, select your three sequences (at the bottom of the page), scroll up a bit and hit the "CLUSTALW" button.

11.	When the alignment finishes click the "Import Alignment" button. This brings you to the "Alignment Tools" page. Select your alignment at the bottom of the page and click the "DrawTree" button. What does this phylogeny tree mean?

12.	Have fun!